Application
Western blot analysis of PARP-1 expression in non-transfected 293T: (A), human PARP-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear and nucleolar localization.Application
Western blot analysis of PARP-1 expression in Jurkat whole cell lysate.| Product Name | PARP-1 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 764-1014 mapping at the C-terminus of PARP-1 of human origin |
| Modification Notes | Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. PARP-2 is part of the base excision repair (BER) pathway, catalyzing the poly(ADP-ribosy)lation of nuclear proteins. PARP-2 is a nuclear, DNA-binding protein which interacts with PARP-1. PARP-2 is present in actively dividing tissues with highest levels in the kidney, skeletal muscle, liver, heart and spleen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PARP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 764-1014 mapping at the C-terminus of PARP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 764-1014 mapping at the C-terminus of PARP-1 of human origin |
| Entrez Gene | 142 |
|---|
Application
Western blot analysis of PARP-1 expression in non-transfected 293T: (A), human PARP-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear and nucleolar localization.
Application
Western blot analysis of PARP-1 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of PARP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
