Application
Western blot analysis of DPYS expression in non-transfected: (A) and human DPYS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human DPYS transfected 293T whole cell lysate.| Product Name | DPYS Antibody (Q-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of DPYS of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | DPYS (dihydropyrimidinase), also known as DHPase, Hydantoinase or DHP, is a 519 amino acid protein that is expressed in liver and kidney tissue and belongs to the DHOase family. Functioning as a homotetramer, DPYS uses zinc as a cofactor to catalyze the second step of reductive pyrimidine degradation, namely the conversion of 5,6-dihydrouracil to 3-ureidopropionate. DPYS is subject to post-translational carbamylation, an event which enhances the ability of DPYS to bind zinc ions. Defects in the gene encoding DPYS are the cause of DHP deficiency, an autosomal recessive disorder that is characterized by epileptic or convulsive attacks, dysmorphic features and severe developmental delay and congenital microvillous atrophy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of DPYS of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of DPYS of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 1807 |
|---|
Application
Western blot analysis of DPYS expression in non-transfected: (A) and human DPYS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human DPYS transfected 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
