Application
Western blot analysis of TRIM14 expression in CCRF-CEM whole cell lysate (A) and mouse thymus tissue extract (B).Application
Western blot analysis of TRIM14 expression in Jurkat whole cell lysate.| Product Name | TRIM14 Antibody (F-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRIM14 of human origin by WB, IP and ELISA |
| Modification Notes | The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM14 (tripartite motif-containing 14) is a 442 amino acid protein belonging to the TRIM/RBCC family. With highest expression in liver, TRIM14 contains one B-box type zinc finger and one B30.2/SPRY domain. Through its B-box zinc finger domain, TRIM14 inhibits the transcriptional activity of PU.1 in a dose-dependent manner. TRIM14 exists as two alternatively spliced isoforms that are designated isoform alpha and isoform beta. TRIM14 is encoded by a gene located on human chromosome 9, which consists of about 145 million bases, 4% of the human genome and encodes nearly 900 genes. Chromosome 9 may play a role in gender determination and deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRIM14 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRIM14 of human origin by WB, IP and ELISA |
| Entrez Gene | 9830 |
|---|
Application
Western blot analysis of TRIM14 expression in CCRF-CEM whole cell lysate (A) and mouse thymus tissue extract (B).
Application
Western blot analysis of TRIM14 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
