Application
Western blot analysis of ZNF514 expression in HeLa whole cell lysate.| Product Name | ZNF514 Antibody (A-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ZNF514 of human origin by WB, IP and ELISA |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF514 (zinc finger protein 514) is a 400 amino acid nuclear protein belonging to the Krüppel C2H2-type zinc-finger protein family. Involved in transcriptional regulation, ZNF514 contains seven C2H2-type zinc fingers, a single KRAB domain, and is encoded by a gene that maps to human chromosome 2q11.1. Human chromosome 2 contains 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2 including Harlequin icthyosis, sitosterolemia and Alström syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ZNF514 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ZNF514 of human origin by WB, IP and ELISA |
| Entrez Gene | 84874 |
|---|
Application
Western blot analysis of ZNF514 expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
