| Product Name | Top3 Antibody (y-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 357-656 mapping at the C-terminus of Top3 of Saccharomyces cerevisiae origin |
| Modification Notes | In budding yeast, loss of topoisomerase III, encoded by the TOP3 gene, leads to a genomic instability phenotype that includes slow growth, hyper-sensitivity to genotoxic agents, mitotic hyper-recombination, increased chromosome missegregation, and meiotic failure. The Saccharomyces cerevisiae TOP3 gene is highly conserved in evolution. The RecQ DNA helicase, yeast Sgs1, forms a complex with topoisomerase III (Top3) and functions during DNA replication to restart forks that have paused due to DNA damage or topological stress. The N-terminal region of Sgs1, which interacts with Top3, is required for complementation of MMS sensitivity and suppression of hyper-recombination in Sgs1 disruptants. Slow growth and other defects of Top3 mutants are suppressed by mutation of Sgs1. Sgs1 is a homologue of the human Bloom’s syndrome and Werner’s syndrome genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Top3 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 357-656 mapping at the C-terminus of Top3 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 357-656 mapping at the C-terminus of Top3 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Top3 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
