Application
Western blot analysis of GTPBP2 expression in non-transfected: (A) and human GTPBP2 transfected: (B) 293T whole cell lysates.| Product Name | GTPBP2 Antibody (U-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GTPBP2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Small G proteins act as molecular switches for regulation of variety of cellular processes, such as nuclear transport, signal transduction, membrane trafficking and protein synthesis. GTPBP2 (GTP-binding protein 2) is a 602 amino acid G protein that is expressed in kidney, skeletal muscle, testis, brain and thymus, though it is not detected in liver. Expression of GTPBP2 is enhanced by ©-interferon stimulation in HeLa cells, THP-1 cells and thioglycollate-elicited mouse peritoneal macrophages. There are four isoforms of GTPBP2 that are expressed as a result of alternative splicing events. Since mutation of the gene encoding GTPBP1 does not lead to any phenotypic abnormalities, it is thought that there may be a genetic redundancy to make up for GTPBP1 lack-of-function. GTPBP2 shares 44% sequence similarity with GTPBP1 and also overlaps in expression pattern, suggesting that the GTPBP2 gene may compensate for GTPBP1 genetic abnormalities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GTPBP2 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of GTPBP2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 54676 |
|---|
Application
Western blot analysis of GTPBP2 expression in non-transfected: (A) and human GTPBP2 transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
