Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of HMGCR expression in non-transfected: (A) and mouse HMGCR transfected: (B) 293T whole cell lysates.| Product Name | HMGCR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 589-888 mapping at the C-terminus of HMGCR of human origin |
| Modification Notes | The human enzyme hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) limits the rate of cholesterol synthesis, a necessary process for cellular growth, in liver tissue. Phosphorylation of HMGCR inactivates the enzyme, which occurs via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from the product of the reductase reaction. Inhibitors of HMGCR (statins) exert anti-inflammatory effects and decrease the frequency of cardiovascular events by lowering plasma cholesterol. Additionally, intermediate products along the pathway catalyzed by HMGCR, which modulate signal transducing proteins such as Ras, provide possible ties between HMGCR regulation and new chemotherapeutic methods. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HMGCR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 589-888 mapping at the C-terminus of HMGCR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 589-888 mapping at the C-terminus of HMGCR of human origin |
| Entrez Gene | 3156 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of HMGCR expression in non-transfected: (A) and mouse HMGCR transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HMGCR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
