| Product Name | DAF-16 Antibody (ce-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 211-510 mapping at the C-terminus of DAF-16 of C. elegans origin |
| Modification Notes | Several proteins involved in regulating the aging process in C. elegans have been identified. DAF-2, DAF-16 and AGE-1 (also known as DAF-23) regulate lifespan via an insulin-signaling pathway (1-3). In specific, decreases in DAF-2 signaling induce metabolic and developmental changes, as in mammalian metabolic control by the insulin receptor (1). DAF-16 encodes a member of the hepatocyte nuclear factor 3 (HNF-3)/forkhead family of transcriptional regulators (2). In humans HNF-3 activity is antagonized by insulin, causing the down regulation of developmental genes, raising the possibility that aspects of the DAF-16 regulatory system have been conserved (2). The gene AGE-1 encodes a homologue of mammalian phosphatidylinositol-3-OH kinase (PI(3)K) catalytic subunits and is required for non-dauer development and normal senescence (3). CLK-1, a homolog of the yeast COQ7/CAT5 protein, is thought to exert its effects on longevity via the synthesis of ubiquinone, an essential component of electron transport (4-7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DAF-16 of C. elegans origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-510 mapping at the C-terminus of DAF-16 of C. elegans origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-510 mapping at the C-terminus of DAF-16 of C. elegans origin |
| Application Notes | recommended for detection of DAF-16 of C. elegans origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
