Application
Western blot analysis of HDAC10 expression in non-transfected: (A) and mouse HDAC10 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HDAC10 expression in A375 whole cell lysate.Application
Western blot analysis of HDAC10 expression in non-transfected (A) and human HDAC10 transfected (B) 293 whole cell lysates.| Product Name | HDAC10 Antibody (Y-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HDAC10 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Histone deacetylases (HDACs) play an important role in the modification of chromatin structure and thus in the suppression and activation of transcription and cellular differentiation. There are 11 members in the HDAC family that are divided into four classes. Class I HDACs represent homologs of the yeast histone deacetylase Rpd3, class II HDACs share strong homology with the yeast histone deacetylase Hda1, class III HDACs are closely related to the yeast Sir2 protein and class IV HDACs comprise histone deacetylase 11 (HDAC11)-related enzymes. HDAC10, also known as HD10, is a member of the class II HDACs. It contains an N-terminal Hda1p-related catalytic domain and a unique C-terminal leucine-rich domain. HDAC10 is ubiquitously expressed and can shuttle between the cytoplasm and nucleus in response to celllular signals. It is able to repress transcription and, like other class II HDAC members, its enzymatic activity is inhibited by Trichostatin A (TSA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HDAC10 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HDAC10 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 83933 |
|---|
Application
Western blot analysis of HDAC10 expression in non-transfected: (A) and mouse HDAC10 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HDAC10 expression in A375 whole cell lysate.
Application
Western blot analysis of HDAC10 expression in non-transfected (A) and human HDAC10 transfected (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
