Application
Western blot analysis of Ku-70 expression in A-431 (A), C32 (B), Jurkat (C), MCF7 (D) nuclear extracts and HeLa whole cell lysate (E).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Ku-70 expression in HeLa (A) and C32 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Product Name | Ku-70 Antibody (H-308) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 302-609 of the 70 kDa subunit of the Ku protein of human origin |
| Modification Notes | The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (p70) and Ku-86 (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ku-70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 302-609 of the 70 kDa subunit of the Ku protein of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 302-609 of the 70 kDa subunit of the Ku protein of human origin |
| Entrez Gene | 2547 |
|---|
Application
Western blot analysis of Ku-70 expression in A-431 (A), C32 (B), Jurkat (C), MCF7 (D) nuclear extracts and HeLa whole cell lysate (E).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Ku-70 expression in non-transfected 293T: (A), human Ku-70 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Ku-70 expression in HeLa (A) and C32 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Application Notes | recommended for detection of Ku-70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
