Application
Western blot analysis of IFN-α/βRα phosphorylation in immuno-precipitates from HEK 293 cells transfected with mock vector (A,C,E) and IFN-α/βRα (B,D,F). Blots were probed with p-IFN-α/βRα (Ser 535/Ser 539): (A,B), p-IFN-a/bRa (Ser 535/Ser 539): preincubated with its cognate phosphorylated peptide (C,D) and p-IFN-α/βRα (Ser 535/Ser 539): preincubated with the corresponding unphosphorylated peptide (E,F).| Product Name | p-IFN-α/βRα Antibody (Ser 535/Ser 539) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 535 and Ser 539 dually phosphorylated IFN-α/βRα of human origin, correspondingly Ser 526 and Ser 530 dually phosphorylated IFN-α/βRα of mouse origin and correspondingly Ser 375 and Ser 379 |
| Modification | Phosphoralated |
| Modification Notes | The type I interferons (IFNs), å and ∫, are a group of structurally and functionally related proteins that are induced by either viruses or double stranded RNA, and defined by their ability to confer an antiviral state in cells. The å and ∫ IFNs appear to compete with one another for binding to a common cell surface receptor. Components of the receptor for IFN-å and -∫ include a 331 amino acid transmembrane glycoprotein, designated IFN-å/∫R and a 557 amino acid component designated IFN-åR. The latter protein, IFN-åR, is weakly responsive to type I interferons in contrast to IFN-å/∫R, which binds to and responds effectively to IFN-∫ and to several of the IFN-å subtypes. Treatment of cells with IFN-å causes the IFN-åR1 subunit of the IFN-å receptor to become phosphorylated at Tyr 466. The region surrounding phosphorylated Tyr 466 binds the SH2 domain of Stat2, facilitating its phosphorylation and thus enhancing IFN-å signal transduction. Mouse, rat and human IFN-å/∫Rå are phosphorylated upon ligand binding on Ser 535 and Ser 539. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| See 1 citations for p-IFN-α/βRαAntibody: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 535 and Ser 539 dually phosphorylated IFN-&alpha /&beta R&alpha of human origin correspondingly Ser 526 and Ser 530 dually phosphorylated IFN-&alpha /&beta R&alpha of mouse origin and correspondingly Ser 375 and Ser 379 |
|---|---|
| Tissue Specificity | recommended for detection of Ser 535 and Ser 539 dually phosphorylated IFN-α/βRα of human origin, correspondingly Ser 526 and Ser 530 dually phosphorylated IFN-α/βRα of mouse origin and correspondingly Ser 375 and Ser 379 dually phosphorylated IFN-α/βRα of rat origin of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine and canine |
| Entrez Gene | 3454 |
|---|
Application
Western blot analysis of IFN-α/βRα phosphorylation in immuno-precipitates from HEK 293 cells transfected with mock vector (A,C,E) and IFN-α/βRα (B,D,F). Blots were probed with p-IFN-α/βRα (Ser 535/Ser 539): (A,B), p-IFN-a/bRa (Ser 535/Ser 539): preincubated with its cognate phosphorylated peptide (C,D) and p-IFN-α/βRα (Ser 535/Ser 539): preincubated with the corresponding unphosphorylated peptide (E,F).| Application Notes | See 1 citations for p-IFN-α/βRαAntibody: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
