Application
Western blot analysis of phosphorylated NFκB p100 expression in untreated (A) and TNFα-treated (B) MDA-MB435 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-NFκB p100 Antibody (Ser 870) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 870 of NFκB p100 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ˚B sequence in the immunoglobulin ˚ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated NFkB p105 and NFkB p100. The NFkB p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity. Phosphorylation of Serine 866 and 870 are involved in NFkB p100 processing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 870 phosphorylated NFκB p100 of human origin, Ser 869 phosphorylated NFκB p100 of mouse origin, and correspondingly phosphorylated NFκB p100 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 870 of NF&kappa B p100 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 870 of NFκB p100 of human origin |
| Entrez Gene | 4791 |
|---|
Application
Western blot analysis of phosphorylated NFκB p100 expression in untreated (A) and TNFα-treated (B) MDA-MB435 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 870 phosphorylated NFκB p100 of human origin, Ser 869 phosphorylated NFκB p100 of mouse origin, and correspondingly phosphorylated NFκB p100 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
