Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing membrane and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of δENaC expression in SK-N-MC (A), MIA PaCa-2 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Product Name | δENaC Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-310 mapping near the N-terminus of δENaC of human origin |
| Modification Notes | The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that is located on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands, and other organs. ENaC is formed by heteromultimerization of four homologous subunits, å, ∫, © and ∂. The most frequently formed heterotetramer consists of two å, one ∫, and one © subunit, but the å subunit can be replaced by a ∂ subunit. The ∂ENaC subunit exists in two isoforms and is abundantly expressed in brain. In particular, ∂ENaC is exclusively found in pyramidal cells of the cerebral cortex. The C-terminus of all ENaC subunits contains PY motifs, which interact with the ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-function mutations involving the PY motif cause Liddle’s syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption. Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of δENaC of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-310 mapping near the N-terminus of &delta ENaC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-310 mapping near the N-terminus of δENaC of human origin |
| Entrez Gene | 6339 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing membrane and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of δENaC expression in SK-N-MC (A), MIA PaCa-2 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Application Notes | recommended for detection of δENaC of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
