Application
Western blot analysis of Rnf14 expression in non-transfected: (A) and mouse Rnf14 transfected: (B) 293T whole cell lysates.| Product Name | ARA54 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA54 of human origin |
| Modification Notes | Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT). ARA54 contains a RING finger and functions as an AR coactivator. Testis, thymus, spleen, colon, pro-state and uterus express ARA54 at high levels. ARA160 associates with an ATP-dependent chromatin remodeling factor known as the SNF/SWI complex. The FXXLF motif present in AR coactivators mediates their interaction with AR but not their transcription-related activity. The genes encoding human ARA54 and ARA160 map to chromosomes 5q23.3-q31.1 and 3p21-p12, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ARA54 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA54 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA54 of human origin |
| Entrez Gene | 9604 |
|---|
Application
Western blot analysis of Rnf14 expression in non-transfected: (A) and mouse Rnf14 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ARA54 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
