Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed MIA Paca-2 cells showing cytoplasmic localization.Application
Western blot analysis of SUR-1 expression in MIA PaCa-2 (A) and JAR (B) whole cell lysates and rat brain tissue extract (C).| Product Name | SUR-1 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 611-690 mapping within an internal region of SUR-1 of human origin |
| Modification Notes | Both suphonylurea receptor-1 (SUR-1) and sulphonylurea receptor-2 (SUR-2) belong to the ATP-binding cassette superfamily associated with KIR6.x. SUR-1 and KIR6.x proteins are required for the regulation of glucose-induced Insulin secretion by controlling K-ATP channel activity of the pancreatic ∫-cell membrane while SUR-2 and KIR6.x proteins reconstitute the cardiac and the vascular-smooth-muscle-type K-ATP channels. Loss-of-function mutations in the SUR-1 gene causes the disease persistent hyperinsulinemic hypoglycemia of infancy (PHHI). PHHI is characterized by increased irregular Insulin secretion, which causes disorganized formation of new islets and leads to hypoglycemia, coma and severe brain damage. The K-ATP channels controlled by SUR-2 are activated during myocardial ischemia, which suggests that mutations in the SUR-2 gene may cause channel malfunction and ischemic injury to the heart. No disease has yet been found to be associated with the SUR-2 gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SUR-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 611-690 mapping within an internal region of SUR-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 611-690 mapping within an internal region of SUR-1 of human origin |
| Entrez Gene | 6833 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed MIA Paca-2 cells showing cytoplasmic localization.
Application
Western blot analysis of SUR-1 expression in MIA PaCa-2 (A) and JAR (B) whole cell lysates and rat brain tissue extract (C).| Application Notes | recommended for detection of SUR-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
