Application
Mouse thymus; Aire Green, Keratin 8 Red. Frozen section fixed with paraformaldehyde and subjected to tris antigen retrieval. Also works with acetone fixation or formalin fixed, paraffin sections subjected to antigen retrieval. No staining of aldehyde-fixed tissue without retrieval. Kindly provided by A.G. Farr, University of Washington, and M.C. Ziga University of California Santa Cruz.Application
Western blot analysis of AIRE-1 expression in MIA PaCa-2 whole cell lysate (A) and mouse thymus (B) and rat thymus (C) tissue extracts.| Product Name | AIRE-1 Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 253-552 mapping at the C-terminus of AIRE-1 of mouse origin |
| Modification Notes | The autoimmune regulator gene, which is defective in the hereditary auto-immune disease APECED, encodes the transcriptional activator AIRE. AIRE is expressed in the medullary epithelial cells and monocyte-dendritic cells of the thymus, with lower expression in the spleen, fetal liver and lymph nodes. In adult tissue, AIRE expression in the thymus is confined to the medulla and the cortico-medullary junction, where it is modulated by thymocytes undergoing negative selection. At the cellular level, AIRE is located in microtubular structures of the cyto-skeleton and in discrete nuclear dots resembling ND10 nuclear bodies. AIRE is induced by developing early thymocytes and is associated with the correct establishment of a regular thymic environment. AIRE regulates thymic architecture via transcriptional control of downstream target genes. AIRE mutations in APECED patients may affect thymic T cell selection and the formation of self-tolerance. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all AIRE-1 isoforms of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 253-552 mapping at the C-terminus of AIRE-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 253-552 mapping at the C-terminus of AIRE-1 of mouse origin |
| Entrez Gene | 326 |
|---|
Application
Mouse thymus; Aire Green, Keratin 8 Red. Frozen section fixed with paraformaldehyde and subjected to tris antigen retrieval. Also works with acetone fixation or formalin fixed, paraffin sections subjected to antigen retrieval. No staining of aldehyde-fixed tissue without retrieval. Kindly provided by A.G. Farr, University of Washington, and M.C. Ziga University of California Santa Cruz.
Application
Western blot analysis of AIRE-1 expression in MIA PaCa-2 whole cell lysate (A) and mouse thymus (B) and rat thymus (C) tissue extracts.| Application Notes | recommended for detection of all AIRE-1 isoforms of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
