Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and human SURF-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SURF-1 expression in non-transfected 293T: (A), human SURF-1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and human SURF-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SURF-1 expression in HeLa whole cell lysate.Application
Western blot analysis of SURF-1 expression in non-transfected 293T: (A), human SURF-1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and SURF-1 transfected: (B) CHO whole cell lysates.| Product Name | SURF-1 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-250 mapping near the C-terminus of SURF-1 of human origin |
| Modification Notes | The SURF-1 protein demonstrates a vital role in the assembly of complex IV (CIV or COX) of the mitochondrial respiratory chain. Expressed in the inner mitochondrial membrane, mutations of the SURF-1 gene generally cause cytochrome c oxidase complex IV deficiency. Shortage of complex IV leads to Leigh syndrome, a severe neurological disorder. Leigh syndrome patients are usually subject to rapidly progressive encephalopathy, characterized by necrotic lesions in subcortical brain regions. SURF-1 mutations correlate to high post-implantation embryonic lethality as well as early-onset mortality of post-natal individuals. Considerable deficit in muscle strength and motor performance is also a profound and isolated defect of SURF-1 activity in skeletal muscle and liver. Heart, brain and skeletal muscle morphological abnormalities frequently occur due to SURF-1 mutations. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SURF-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, canine and equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-250 mapping near the C-terminus of SURF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-250 mapping near the C-terminus of SURF-1 of human origin |
| Entrez Gene | 6834 |
|---|
Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and human SURF-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SURF-1 expression in non-transfected 293T: (A), human SURF-1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and human SURF-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SURF-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of SURF-1 expression in non-transfected 293T: (A), human SURF-1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of SURF-1 expression in non-transfected: (A) and SURF-1 transfected: (B) CHO whole cell lysates.| Application Notes | recommended for detection of SURF-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, canine and equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
