Application
Western blot analysis of MCP-1-4/eotaxin expression in transfected: (A) and MCP-1 non-transfected: (B) whole cell lysates.| Product Name | MCP-1-4/eotaxin Antibody (FL-99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-99 representing full length MCP-1 of human origin |
| Modification Notes | Eotaxin and the monocyte chemotactic proteins, MCP-1–5, form a subfamily of the C-C (or ∫) chemokines, which are characterized by a set of conserved adjacent cysteines. MCPs are produced by a variety of cells, including T lymphocytes, subsequent to their activation with cytokines such as IL-1, TNFå and IFN-©. In vitro studies have shown that the MCP isoforms exhibit their chemotactic effects on different subpopulations of lymphocytes. MCP-1 is a potent basophil activator but does not affect eosinophils. MCP-1 levels are increased during infection and inflammation, which are both characterized by leukocyte infiltration. Two MCP-1 receptors, which differ in their carboxy-termini, have been identified. MCP-2 stimulates both eosinophils and basophils, while eotaxin serves as a potent chemo-attractant for eosinophils. MCP-3 has been shown to have the broadest range of influence, activating monocytes, dendritic cells, lymphocytes, NK cells, eosinophils, basophils and neutrophils. MCP-4 signals through CCR-2 and -3 and is a potent chemoattractant for monocytes, eosinophils and basophils induced in allergic and nonallergic inflammation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MCP-1, 2, 3, 4 and eotaxin of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-99 representing full length MCP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-99 representing full length MCP-1 of human origin |
| Entrez Gene | 6347 |
|---|
Application
Western blot analysis of MCP-1-4/eotaxin expression in transfected: (A) and MCP-1 non-transfected: (B) whole cell lysates.| Application Notes | recommended for detection of MCP-1, 2, 3, 4 and eotaxin of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
