Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of 11β-HSD2 expression in non-transfected 293T: (A), human 11β-HSD2 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | 11β-HSD2 Antibody (H-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-405 mapping at the C-terminus of 11β-HSD2 of human origin |
| Modification Notes | Glucocorticoid hormone action in target tissues is modulated by 11b-hydroxysteroid dehydrogenase (11b-HSD), which catalyzes the interconversion of hormonally active C11-hydroxylated corticosteroids (cortisol, corticosterone) and their inactive C11-keto metabolites (cortisone, 11-dehydrocorticosterone) (1,2). At least two isoforms of 11b-HSD exist: a low-affinity NADP-dependent dehydrogenase/oxoreductase (11b-HSD1) and a high-affinity NAD-dependent dehydrogenase (11b-HSD2) (2). The glycosylated 11b-HSD1 protein activates cortisol from cortisone, which is widely expressed in mammals, and is most highly expressed in the liver. 11b-HSD2 inactivates cortisol to cortisone and is expressed in placenta, aldosterone target tissues (kidney, parotid, colon and skin) and pancreas (3–5). 11b-HSD1 may play a role in glucose homeostasis and pathogenesis of a number of disorders including insulin resistance and obesity (6,7). 11b-HSD2 associates with differentiation or maturation in human colonic epithelia and may serve as a marker in development and disease (8). In addition, 11b-HSD2 plays a crucial role in modulating mineralcorticoid and glucocorticoid receptor occupancy by glucocorticoids (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of 11β-HSD2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-405 mapping at the C-terminus of 11&beta -HSD2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-405 mapping at the C-terminus of 11β-HSD2 of human origin |
| Entrez Gene | 3291 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of 11β-HSD2 expression in non-transfected 293T: (A), human 11β-HSD2 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of 11β-HSD2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
