Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of Rab 11B expression in non-transfected 293T: (A), mouse Rab 11B transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Rab 11A expression in non-transfected: (A) and mouse Rab 11A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rab 11 expression in non-transfected: (A) and mouse Rab 11 transfected: (B) CHO whole cell lysates.Application
Western blot analysis of Rab 11 expression in platelet (A), Rab 11 non-transfected CHO: (B) and Rab 11 transfected CHO: (C) whole cell lysates.Application
Western blot analysis of Rab 11 expression in human platelet whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing perinuclear and nuclear localization.Application
Western blot analysis of human recombinant Rab 11.| Product Name | Rab 11 Antibody (H-87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 130-216 of Rab 11A of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21 (1). Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport (1,2). The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function (1-6). The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles (9). Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway (3-8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rab 11A and, to a lesser extent, RAB 11B isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 130-216 of Rab 11A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 130-216 of Rab 11A of human origin |
| Entrez Gene | 8766 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of Rab 11B expression in non-transfected 293T: (A), mouse Rab 11B transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Rab 11A expression in non-transfected: (A) and mouse Rab 11A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 11 expression in non-transfected: (A) and mouse Rab 11 transfected: (B) CHO whole cell lysates.
Application
Western blot analysis of Rab 11 expression in platelet (A), Rab 11 non-transfected CHO: (B) and Rab 11 transfected CHO: (C) whole cell lysates.
Application
Western blot analysis of Rab 11 expression in human platelet whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing perinuclear and nuclear localization.
Application
Western blot analysis of human recombinant Rab 11.| Application Notes | recommended for detection of Rab 11A and, to a lesser extent, RAB 11B isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
