Application
Western blot analysis of α-Dystrobrevin expression in 293T whole cell lysate.Application
Western blot analysis of α-Dystrobrevin expression in non-transfected 293T: (A), mouse α-Dystrobrevin transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of Dystrobrevin expression in SK-N-SH (A) and C2C12 (B) whole cell lysates.| Product Name | α-Dystrobrevin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 301-600 (deletion 366-422) mapping within an internal region of α-Dystrobrevin of human origin |
| Modification Notes | Dystrobrevins are protein components of the dystrophin complex, whose disruption leads to Duchenne muscular dystrophy and related diseases. å-dystrobrevin is a dystrophin-related and -associated protein that is involved in synapse maturation and is required for normal muscle function. å-Dystrobrevin is a component of the dystrophin glycoprotein complex. It is localized to the cytoplasmic side of the sarcolemma and is highly concentrated at the neuromuscular junctions in skeletal muscle. The insertion of 57 amino acids by alternative splicing accounts for the increase in molecular mass of å-Dystrobrevin 1 in skeletal and cardiac muscle compared with brain and lung. å-Dystrobrevin containing complexes are found in endothelial and smooth muscle cells, while ∫-Dystrobrevin containing complexes are present at the basal region of renal epithelial cells. Additionally, ∫-Dystrobrevin is found in neurons and is highly enriched in postsynaptic densities. Alternative splicing of å-Dystrobrevin produces ©-Dystrobrevin (isoform 5), ∂-Dystrobrevin (isoform 7), é-Dystrobrevin (isoform 6) and Ω-Dystrobrevin (isoform 8). Additional isoforms may also exist. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all isoforms of α-Dystrobrevin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 301-600 (deletion 366-422) mapping within an internal region of &alpha -Dystrobrevin of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 301-600 (deletion 366-422) mapping within an internal region of α-Dystrobrevin of human origin |
| Entrez Gene | 1837 |
|---|
Application
Western blot analysis of α-Dystrobrevin expression in 293T whole cell lysate.
Application
Western blot analysis of α-Dystrobrevin expression in non-transfected 293T: (A), mouse α-Dystrobrevin transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of Dystrobrevin expression in SK-N-SH (A) and C2C12 (B) whole cell lysates.| Application Notes | recommended for detection of all isoforms of α-Dystrobrevin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
