| Product Name | NEPH1 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 608-757 mapping within a C-terminal cytoplasmic domain of NEPH1 of human origin |
| Modification Notes | Glomerular visceral epithelial cells, also known as podocytes, maintain the selective filtration barrier of the renal glomerulus. NEPH1, a member of the immunoglobulin superfamily, plays a critical role in functional barrier development. Loss of NEPH1 expression, like that of its structural relative nephrin, results in nephrotic syndromes and proteinuria leading to perinatal death. NEPH1 associates with nephrin as well as ZO-1 and localizes with them to the glomerular slit diaphragm. Interaction with nephrin occurs via the extracellular domain of NEPH1 and with ZO-1 in a PDZ binding motif of the cytoplasmic tail. Mutation of a putative threonine phosphorylation site within the cytoplasmic domain abrogates interaction with ZO-1, implying that phosphorylation regulates this interaction, and may effect the recruitment of the appropriate signal transduction components to the complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEPH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 608-757 mapping within a C-terminal cytoplasmic domain of NEPH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 608-757 mapping within a C-terminal cytoplasmic domain of NEPH1 of human origin |
| Entrez Gene | 55243 |
|---|
| Application Notes | recommended for detection of NEPH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
