Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of SOD-2 expression in non-transfected 293T: (A), human SOD-2 transfected 293T: (B) and HISM (C) whole cell lysates.Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of SOD-2 expression in DU 145 (A), HISM (B), SK-N-MC (C) and T98G (D) whole cell lysates.| Product Name | SOD-2 Antibody (FL-222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-222 representing full length SOD-2 of human origin |
| Modification Notes | The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SOD-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-222 representing full length SOD-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-222 representing full length SOD-2 of human origin |
| Entrez Gene | 6648 |
|---|
Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of SOD-2 expression in non-transfected 293T: (A), human SOD-2 transfected 293T: (B) and HISM (C) whole cell lysates.
Application
Western blot analysis of SOD-2 expression in non-transfected: (A) and human SOD-2 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of SOD-2 expression in DU 145 (A), HISM (B), SK-N-MC (C) and T98G (D) whole cell lysates.| Application Notes | recommended for detection of SOD-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
