Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.Application
Western blot analysis of HRF expression in non-transfected 293T: (A), human HRF transfected 293T: (B) and DU 145 (C) whole cell lysates.Application
Western blot analysis of HRF expression in non-transfected 293: (A), human HRF transfected 293: (B) and DU 145 (C) whole cell lysates.Application
Western blot analysis of HRF expression in human HRF transfected: (A) and non-transfected: (B) 293 whole cell lysates.Application
Western blot analysis of HRF expression in DU 145 (A) and CCRF-CEM (B) whole cell lysates.| Product Name | HRF Antibody (FL-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-172 representing full length HRF of human origin |
| Modification Notes | Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases and, due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer’s disease and Down syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HRF and FKSG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-172 representing full length HRF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-172 representing full length HRF of human origin |
| Entrez Gene | 7178 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.
Application
Western blot analysis of HRF expression in non-transfected 293T: (A), human HRF transfected 293T: (B) and DU 145 (C) whole cell lysates.
Application
Western blot analysis of HRF expression in non-transfected 293: (A), human HRF transfected 293: (B) and DU 145 (C) whole cell lysates.
Application
Western blot analysis of HRF expression in human HRF transfected: (A) and non-transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of HRF expression in DU 145 (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of HRF and FKSG2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
