Application
Western blot analysis of GGA1 expression in non-transfected: (A) and human GGA1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GGA1 expression in non-transfected 293T: (A), mouse GGA1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | GGA1 Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 286-500 mapping within an internal region of GGA1 of human origin |
| Modification Notes | The GGA family of proteins (Golgi-localized, ©-adaptin ear-containing, ARF-binding proteins) are ubiquitous coat proteins that facilitate the trafficking of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ARF (ADP-ribosylation factor), and clathrin (1–3). Members of the GGA family, GGA1,GGA2 (also known as VEAR) and GGA3, are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) (1,2,4). GGAs have modular structures with an N-terminal VHS (VPS-27, Hrs, and STAM) domain followed by a GAT (GGA and TOM1) domain, a connecting hinge segment, and a C-terminal GAE (©-adaptin ear) domain (5). The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster di-leucine (ACLL) sequences (3). GGA1 and GGA2 do not associate with each other, but they do colocalize on perinuclear membranes (2). The cytosolic domain of memapsin 2, but not that of memapsin 1, binds the VHS domains of GGA1 and GGA2 (6). The human GGA1 gene maps to chromosome 22 and encodes a protein that shares 45% sequence identity with GGA2 and GGA3 (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GGA1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 286-500 mapping within an internal region of GGA1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 286-500 mapping within an internal region of GGA1 of human origin |
| Entrez Gene | 26088 |
|---|
Application
Western blot analysis of GGA1 expression in non-transfected: (A) and human GGA1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GGA1 expression in non-transfected 293T: (A), mouse GGA1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of GGA1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
