Application
Western blot analysis of γ-GCSc expression in non-transfected 293T: (A), human γ-GCSc transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of -GCSc expression in non-transfected: (A) and human -GCSc transfected: (B) 293T whole cell lysates.Application
Western blot analysis of γ-GCSc expression in non-transfected: (A) and mouse γ-GCSc transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | γ-GCSc Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 338-637 mapping at the C-terminus of γ-GCSc of human origin |
| Modification Notes | The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GSH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 338-637 mapping at the C-terminus of &gamma -GCSc of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 338-637 mapping at the C-terminus of γ-GCSc of human origin |
| Entrez Gene | 2729 |
|---|
Application
Western blot analysis of γ-GCSc expression in non-transfected 293T: (A), human γ-GCSc transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of -GCSc expression in non-transfected: (A) and human -GCSc transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of γ-GCSc expression in non-transfected: (A) and mouse γ-GCSc transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of GSH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
