Application
Western blot analysis of Septin 6 expression in non-transfected 293T: (A), mouse Septin 6 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed CCRF-CEM cells showing cytoplasmic localization.Application
Western blot analysis of Septin 6 expression in K-562 (A) CCRF-CEM (B) and MOLT-4 (C) whole cell lysates.| Product Name | Septin 6 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 365-424 mapping at the C-terminus of Septin 6 of human origin |
| Modification Notes | Septin 6, also known as SEPT6, is a 434 amino acid protein that belongs to the septin family. The highly conserved septin family of GTP-binding cytoskeletal proteins is implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis and other cellular functions. Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. Filaments are assembled from asymmetrical heterotrimers, composed of Septin 2, Septin 6 and Septin 7 that associate head-to-head to form a hexameric unit, however interaction with Septin 12 alters filament structure. Septin 6 shares 40% identity with CDC10 and contains an ATP/GTP-binding site motif. The Septin 6 protein demonstrates nearly ubiquitous expression, with highest level in thymus. The Septin 6 protein is phosphorylated upon DNA damage, probably by Atm or ATR.Existing as four alternatively spliced isoforms, the Septin 6 gene is conserved in dog, cow, mouse, rat, chicken, zebrafish and fruit fly, and maps to human chromosome X. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Septin 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 365-424 mapping at the C-terminus of Septin 6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 365-424 mapping at the C-terminus of Septin 6 of human origin |
| Entrez Gene | 23157 |
|---|
Application
Western blot analysis of Septin 6 expression in non-transfected 293T: (A), mouse Septin 6 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed CCRF-CEM cells showing cytoplasmic localization.
Application
Western blot analysis of Septin 6 expression in K-562 (A) CCRF-CEM (B) and MOLT-4 (C) whole cell lysates.| Application Notes | recommended for detection of Septin 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
