Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Dbl expression in NIH/3T3 whole cell lysate.| Product Name | Dbl Antibody (M-138) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 791-928 mapping at the C-terminus of Dbl of mouse origin |
| Modification Notes | The superfamily of GTP binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of a broad range of biological activities . One member of the family, Cdc42Hs (originally referred to as Gp or G25K), appears to represent the human homolog of the Saccharomyces cerevisiae cell division protein, Cdc42Sc. The predicted amino acid sequence of Cdc42Hs is very similar to those of various members of the Ras superfamily proteins including N-, K- and H-Ras proteins (30-35% identical), Rho proteins (50% identical) and the Rac proteins (70% identical). A second S. cerevisiae protein, Cdc24, which is known from complementation studies to act with Cdc42Sc to regulate the development of normal cell shape in yeast, contains a region of sequence homology with the Dbl oncogene product. Dbl specifically catalyzes the dissociation of GDP from Cdc42Hs, thus representing a highly selective guanine nucleotide exchange factor for Cdc42Hs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dbl of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 791-928 mapping at the C-terminus of Dbl of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 791-928 mapping at the C-terminus of Dbl of mouse origin |
| Entrez Gene | 109904 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Dbl expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Dbl of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
