Application
Immunofluorescence staining of normal mouse spleen frozen section showing cytoplasmic staining.Application
Western blot analysis of spectrin α II expression in WI 38 (A) and SK-N-SH (B) whole cell lysates and rat brain tissue extract (C).| Product Name | spectrin α II Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2368-2472 mapping at the C-terminus of spectrin β II of human origin |
| Modification Notes | Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous å and ∫ chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin å I and spectrin ∫ I are present in erythrocytes, whereas spectrin å II (also designated fodrin å) and spectrin ∫ II (also designated fodrin ∫) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin å II, a neuronal cytoskeleton protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of spectrin α II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2368-2472 mapping at the C-terminus of spectrin &beta II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2368-2472 mapping at the C-terminus of spectrin β II of human origin |
| Entrez Gene | 6709 |
|---|
Application
Immunofluorescence staining of normal mouse spleen frozen section showing cytoplasmic staining.
Application
Western blot analysis of spectrin α II expression in WI 38 (A) and SK-N-SH (B) whole cell lysates and rat brain tissue extract (C).| Application Notes | recommended for detection of spectrin α II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
