Application
Western blot analysis of IRP-1 expression in non-transfected: (A) and mouse IRP-1 transfected: (B) 293T whole cell lysates.| Product Name | IRP-1 Antibody (H-64) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 826-889 of IRP-1 of human origin |
| Modification Notes | Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins; iron regulatory protein-1 and -2 (IRP-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE–BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IRP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 826-889 of IRP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 826-889 of IRP-1 of human origin |
| Entrez Gene | 48 |
|---|
Application
Western blot analysis of IRP-1 expression in non-transfected: (A) and mouse IRP-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IRP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
