Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | G6Pase-α Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-60 mapping at the N-terminus of G6Pase-α of human origin |
| Modification Notes | Glucose-6-phosphatase (G6Pase), is a multicomponent enzyme system that hydrolyzes glucose-6-phosphate (G6P) in the final step of gluconeogenesis and gluconeolysis. G6Pase localizes to the endoplasmic reticulum, and while liver, kidney, and intestine are the only tissues that express the first identified isoform, G6Pase-å, a second form, designated G6Pase-∫, contributes to blood glucose homeostasis in a wider range of tissues. Glucocorticoids stimulate the expression of the G6Pase gene while Insulin rapidly inhibits expression via the thymine-rich Insulin response element located within the promoter of the G6Pase gene. Due to its necessary involvement in normal glucose metabolism, G6Pase plays an integral role in Diabetes and glycogen storage diseases (GSDs). The presence of different isoforms may explain the ability of some individuals with GSDs to still produce glucose, despite their lack of functional G6Pase-å. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of G6Pase-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 mapping at the N-terminus of G6Pase-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 mapping at the N-terminus of G6Pase-α of human origin |
| Entrez Gene | 2538 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of G6Pase-α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
