Application
Western blot analysis of Chfr expression in ES-2 whole cell lysate.| Product Name | Chfr Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 324-623 mapping at the C-terminus of Chfr of human origin |
| Modification Notes | The forkhead-associated (FHA) domain was initially identified in transcription factors that have forkhead DNA-binding domains and in protein kinases, but many cell-cycle checkpoint proteins, including chfr (checkpoint with forkhead and ring finger domains) contain FHA domains. Chfr defines a checkpoint that delays entry into metaphase in response to mitotic stress. Normal primary cells and tumor cell lines that express wild-type chfr exhibit delayed entry into metaphase when centrosome separation is inhibited by mitotic stress. Additionally, chfr seems to be required for delaying prophase in human cells. The sequence of chfr is similar to that of the fission yeast DMA1, which is involved in a later mitotic checkpoint that delays a cell's exit from mitosis in response to spindle damage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Chfr of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 324-623 mapping at the C-terminus of Chfr of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 324-623 mapping at the C-terminus of Chfr of human origin |
| Entrez Gene | 55743 |
|---|
Application
Western blot analysis of Chfr expression in ES-2 whole cell lysate.| Application Notes | recommended for detection of Chfr of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
