Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cere-bellum tissue showing cytoplasmic staining of Purkinje cells.Application
Western blot analysis of MAD2 expression in HeLa (A), BJAB (B), MEG-01 (C) and Jurkat (D) nuclear extracts.| Product Name | MAD2 Antibody (FL-205) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-205 representing full length MAD2 of human origin |
| Modification Notes | Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MAD2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-205 representing full length MAD2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-205 representing full length MAD2 of human origin |
| Entrez Gene | 4085 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cere-bellum tissue showing cytoplasmic staining of Purkinje cells.
Application
Western blot analysis of MAD2 expression in HeLa (A), BJAB (B), MEG-01 (C) and Jurkat (D) nuclear extracts.| Application Notes | recommended for detection of MAD2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
