Application
Antibodies tested include p-ErbB-3 (Tyr 1328): (A,B,C) and ErbB-3 (5A12): (D,E,F).Application
Western blot analysis of phosphorylated ErbB-3 expression in untreated (A) and EGF-treated (B) HUVEC cell extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.| Product Name | p-ErbB-3 Antibody (Tyr 1328) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 1328 phosphorylated ErbB-3 of human origin and correspondingly phosphorylated Tyr 1325 of mouse and rat origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3. Mouse, rat and human ErbB-3 are subject to phosphorylation on Tyr 1328, an event which is increased by ligand binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 1328 phosphorylated ErbB-3 of human origin and correspondingly phosphorylated Tyr 1325 of mouse and rat origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 1328 phosphorylated ErbB-3 of human origin and correspondingly phosphorylated Tyr 1325 of mouse and rat origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 2065 |
|---|
Application
Antibodies tested include p-ErbB-3 (Tyr 1328): (A,B,C) and ErbB-3 (5A12): (D,E,F).
Application
Western blot analysis of phosphorylated ErbB-3 expression in untreated (A) and EGF-treated (B) HUVEC cell extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
