Application
Western blot analysis of 4E-BP1 expression in non-transfected: (A) and human 4E-BP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 4E-BP1 expression in non-transfected: (A) and mouse 4E-BP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 4E-BP1 expression in A-431 (A), K-562 (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of 4E-BP1 expression in 3T3-L1 whole cell lysate.Application
Antibodies tested include 4E-BP1 (N-19): (A), 4E-BP1 (C-19): (B) and 4E-BP1 (R-113): (C).| Product Name | 4E-BP1 Antibody (R-113) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-117 representing full length rat 4E-BP1 |
| Modification Notes | The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of translational repressor 4E-BP1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with 4E-BP2 and 4E-BP3 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-117 representing full length rat 4E-BP1 |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-117 representing full length rat 4E-BP1 |
| Entrez Gene | 1978 |
|---|
Application
Western blot analysis of 4E-BP1 expression in non-transfected: (A) and human 4E-BP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 4E-BP1 expression in non-transfected: (A) and mouse 4E-BP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 4E-BP1 expression in A-431 (A), K-562 (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of 4E-BP1 expression in 3T3-L1 whole cell lysate.
Application
Antibodies tested include 4E-BP1 (N-19): (A), 4E-BP1 (C-19): (B) and 4E-BP1 (R-113): (C).| Application Notes | recommended for detection of translational repressor 4E-BP1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with 4E-BP2 and 4E-BP3 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
