Application
Western blot analysis of Sialyltransferase 7F expression in Hep G2 whole cell lysate.Application
Western blot analysis of Sialyltransferase 7F expression in non-transfected: (A) and mouse Sialyltransferase 7F transfected: (B) 293T whole cell lysates.| Product Name | Sialyltransferase 7F Antibody (E-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Sialyltransferase 7F of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Sialyltransferase 7F, also known as SIAT7F and ST6GalNAc VI, is a 333 amino acid Golgi type II transmembrane glycosyltransferase expressed in the proximal tubule epithelial cells of kidney. Sialtransferase 7F belongs to the ST6GalNAc family of sialyltransferases involved in the biosynthesis of å-series gangliosides. Gangliosides are glycosphingolipids with sialic acids in the carbohydrate portion and are critical components to a variety of cellular events including cell adhesion, protein targeting, cell-cell interation and mediation of invasion of vectors. Sialyltransferase 7F acts on the substrates GD1a, GT1b and GM1b, and is responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in kidney. In addition, Sialyltransferase 7E can catalyze the synthesis of Disialyl Lc4 from Sialyl Lc4, leading to the synthesis of disialyl Lewis a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Sialyltransferase 7F of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Sialyltransferase 7F of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 30815 |
|---|
Application
Western blot analysis of Sialyltransferase 7F expression in Hep G2 whole cell lysate.
Application
Western blot analysis of Sialyltransferase 7F expression in non-transfected: (A) and mouse Sialyltransferase 7F transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
