Application
Western blot analysis of Sam 68 expression in non-transfected 293T: (A), mouse Sam 68 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Sam 68 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Sam 68 (C-20): .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.Application
Western blot analysis of Sam 68 expression in A-431 whole cell lysate (A), A-431 nuclear extract (B) and HeLa (C) and NIH/3T3 (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Product Name | Sam 68 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Sam 68 of human origin |
| Modification Notes | Sam 68 is a protein that is phosphorylated on tyrosine and functions as a substrate for Src family tyrosine kinases during mitosis. Sam 68 also associates with several SH2 and SH3 domain-containing signaling proteins, such as GRB2 and PLC ©1. Originally cloned as Ras GAP-associated p62, further investigations have shown that Sam 68 and Ras GAP-associated p62 are not antigenically related, nor are they encoded by the same gene. Like Sam 68, the Sam 68-like mammalian proteins, SLM-1 and SLM-2, demonstrate RNA binding activity. Also like Sam 68, SLM-1 is tyrosine phosphorylated and functions as an adapter protein for signaling molecules, including GRB2, PLC ©1, Fyn and Ras GAP. SLM-2 is not tyrosine phosphorylated, nor does it appear to associate with GRB2, PLC ©1, Fyn or Ras GAP, indicating that SLM-2 may not be an adapter protein for these proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sam 68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Sam 68 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Sam 68 of human origin |
| Entrez Gene | 10657 |
|---|
Application
Western blot analysis of Sam 68 expression in non-transfected 293T: (A), mouse Sam 68 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Sam 68 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Sam 68 (C-20): .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.
Application
Western blot analysis of Sam 68 expression in A-431 whole cell lysate (A), A-431 nuclear extract (B) and HeLa (C) and NIH/3T3 (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.| Application Notes | recommended for detection of Sam 68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
