Application
Western blot analysis of Neu phosphorylation in untreated (A) and lambda protein phosphatase treated (B) SK-BR-3 whole cell lysates.Application
Western blot analysis of Neu phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MDA-MB-231 whole cell lysates.Application
Western blot analysis of Neu phosphorylation in EGF treated (A) and EGF and lambda protein phosphatase treated (B) A-431 whole cell lysates.Application
Blots were probed with p-Neu (Tyr 1221/1222): preincubated with cognate phosphorylated peptide (A) and p-Neu (Tyr 1221/1222): (B).Application
Antibodies tested include p-Neu (Tyr 1221/1222): (A,B,C) and Neu (3B5): (D,E,F).| Product Name | p-Neu Antibody (Tyr 1221/1222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 1221/1222 of Neu of human origin |
| Modification | Phosphoralated |
| Modification Notes | Neu (v-ErbB-2 erythroblastic leukemia viral oncogene homolog 2, HER-2, NGL, TKR1, c-ErbB-2) oncogene was originally cloned from a rat neuroglio-blastoma. Human Neu is referred to as HER2 since the protein structure re-sembles human epidermal growth factor receptor (HER). ErbB-2 refers to a high level of similarity to ErbB (avian erythroblastosis oncogene B), later found to code for EGFR (HER). Tyr 1248 phosphorylated Neu localizes with Mucin4/Sialomucin complex at the apical surfaces of ductal and alveolar cells in rodent lactating gland. Phosphorylation of Neu at Tyr 1139 promotes association of GRB2 and GRB7 through a Src homology 2 (SH2) domain-dependent interaction, and contributes to the etiology of certain breast, gastric and esophageal cancers, and testicular germ cell tumors. Neu phosphorylation on Tyr 1221 and Tyr 1248 promotes association of Shc (SH2 domain-containing transforming protein 1) through an SH2 domain. Neu phosphorylation at Tyr 1196 and Tyr 1248 promotes association of Shc through a PTB (phosphotyrosine binding) domain. SH2 and PTB domains recognize tyrosine phosphorylated proteins in a sequence-specific fashion and transduce extracellular signals via subcellular targeting, directing assembly of complexes and modulating enzymatic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 1221/1222 phosphorylated Neu of h origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 1221/1222 of Neu of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 1221/1222 of Neu of human origin |
| Entrez Gene | 2064 |
|---|
Application
Western blot analysis of Neu phosphorylation in untreated (A) and lambda protein phosphatase treated (B) SK-BR-3 whole cell lysates.
Application
Western blot analysis of Neu phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MDA-MB-231 whole cell lysates.
Application
Western blot analysis of Neu phosphorylation in EGF treated (A) and EGF and lambda protein phosphatase treated (B) A-431 whole cell lysates.
Application
Blots were probed with p-Neu (Tyr 1221/1222): preincubated with cognate phosphorylated peptide (A) and p-Neu (Tyr 1221/1222): (B).
Application
Antibodies tested include p-Neu (Tyr 1221/1222): (A,B,C) and Neu (3B5): (D,E,F).| Application Notes | recommended for detection of Tyr 1221/1222 phosphorylated Neu of h origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
