Application
Western blot analysis of apoB expression in human plasma (A) and mouse plasma (B) whole cell lysates.Application
Western blot analysis of apoB expression in human plasma whole cell lysate.| Product Name | apoB Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of apoB of human origin |
| Modification Notes | Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by Apobec-1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB100, apoB48, is synthesized in the small intestine and contains the amino-terminal 2152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of apoB of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of apoB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of apoB of human origin |
| Entrez Gene | 338 |
|---|
Application
Western blot analysis of apoB expression in human plasma (A) and mouse plasma (B) whole cell lysates.
Application
Western blot analysis of apoB expression in human plasma whole cell lysate.| Application Notes | recommended for detection of apoB of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
