Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of neuroligin 1 expression in HeLa whole cell lysate.| Product Name | neuroligin 1 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 645-689 mapping within an extracellular domain of neuroligin 1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Neuroligins are a family of plasma membrane proteins that possess an N-terminal hydrophobic domain, a large esterase homology domain, a single transmembrane region, a short cytoplasmic domain, and an EF-hand binding domain. Members of the neuroligin family include neuroligin 1, neuroligin 2 and neuroligin 3. Neuroligins are expressed in excitatory neuronal synaptic clefts. Neuroligins play a role in the formation and remodeling of CNS synapses by binding to b-neurexins, a family of neuronal cell surface proteins. Neurexin 1∫ binds to the EF-hand domain of neuroligin 1 and requires calcium ion. Neuroligins also bind to PSD-95, which may recruit ion channels and neurotransmitter receptors to the synapses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 ELISA:1:1000~1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, bovine, porcine and avian |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 645-689 mapping within an extracellular domain of neuroligin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 645-689 mapping within an extracellular domain of neuroligin 1 of human origin |
| Entrez Gene | 22871 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of neuroligin 1 expression in HeLa whole cell lysate.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 ELISA:1:1000~1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
