Application
Western blot analysis of PICK1 expression in non-transfected: (A) and human PICK1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PICK1 expression in non-transfected 293T: (A), human PICK1 transfected 293T: (B) and SH-SY5Y (C) whole cell lysates.Application
Western blot analysis of PICK1 expression in non-transfected: (A) and mouse PICK1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PICK1 expression in non-transfected (A) and human PICK1 transfected (B) 293 whole cell lysates.Application
Western blot analysis of PICK1 expression in SH-SY5Y whole cell lysate.| Product Name | PICK1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PICK1 of human origin |
| Modification Notes | Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCå activation. PKCå, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCå. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PICK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PICK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PICK1 of human origin |
| Entrez Gene | 9463 |
|---|
Application
Western blot analysis of PICK1 expression in non-transfected: (A) and human PICK1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PICK1 expression in non-transfected 293T: (A), human PICK1 transfected 293T: (B) and SH-SY5Y (C) whole cell lysates.
Application
Western blot analysis of PICK1 expression in non-transfected: (A) and mouse PICK1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PICK1 expression in non-transfected (A) and human PICK1 transfected (B) 293 whole cell lysates.
Application
Western blot analysis of PICK1 expression in SH-SY5Y whole cell lysate.| Application Notes | recommended for detection of PICK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
