Application
Western blot analysis of Claspin expression in non-transfected: (A) and mouse Claspin transfected: (B) 293T whole cell lysates.| Product Name | Claspin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1033-1332 mapping at the C-terminus of Claspin of human origin |
| Modification Notes | Claspin, an essential replication checkpoint control protein, regulates the interaction between Chk1 and the upstream regulatory kinase ATR. Chk1 mediates cell cycle arrest in response to a block in DNA replication or to DNA damage by ultraviolet radiation. Claspin becomes phosphorylated within its Chk1 binding domain in response to replication stress. This domain consists of two highly conserved repeats of approximately ten amino acids. Each repeat contains a serine residue (serine 864 and serine 895) that undergoes phosphorylation. Binding of Chk1 and Claspin promotes the interaction between Chk1 and ATR and Rad9, thereby arresting the cell cycle. Claspin is most abundant within cells at the S/G2 phase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Claspin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1033-1332 mapping at the C-terminus of Claspin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1033-1332 mapping at the C-terminus of Claspin of human origin |
| Entrez Gene | 63967 |
|---|
Application
Western blot analysis of Claspin expression in non-transfected: (A) and mouse Claspin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Claspin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
