Application
Western blot analysis of DCAMKL1 expression in non-transfected: (A) and truncated mouse DCAMKL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Doublecortin expression in non-transfected 293T: (A), human Doublecortin transfected 293T: (B) and SK-N-SH (C) whole cell lysates.| Product Name | Doublecortin Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 123-402 mapping at the C-terminus of doublecortin of human origin |
| Modification Notes | Lissencephaly (smooth brain) is an abnormality of brain development characterized by incomplete neuronal migration and a smooth cerebral surface, resulting in severe mental retardation. Genetic analysis identified two proteins that are mutated in some cases of lissencephaly, designated lissencephaly-1 protein (LIS1) and doublecortin. LIS1 shows sequence homology to ß-subunits of heterotrimeric G proteins. Doublecortin contains a consensus Abl phosphorylation site, and it has some sequence homology to a predicted kinase protein. Both proteins are highly expressed in developing brain, suggesting that they may be involved in a signal transduction pathway that is crucial to brain development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of doublecortin and DCAMKL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 123-402 mapping at the C-terminus of doublecortin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 123-402 mapping at the C-terminus of doublecortin of human origin |
| Entrez Gene | 1641 |
|---|
Application
Western blot analysis of DCAMKL1 expression in non-transfected: (A) and truncated mouse DCAMKL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Doublecortin expression in non-transfected 293T: (A), human Doublecortin transfected 293T: (B) and SK-N-SH (C) whole cell lysates.| Application Notes | recommended for detection of doublecortin and DCAMKL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
