Application
Western blot analysis of DYNLL2 expression in non-transfected 293T: (A), mouse DYNLL2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of DYNLL1 expression in non-transfected 293T: (A), human DYNLL1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of DYNLL1/2 expression in HeLa whole cell lysate.Application
Western blot analysis of DYNLL1/2 expression in 293 (A), human platelet (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of DYNLL1/2 expression in HeLa whole cell lysate (A) and mouse testis tissue extract (B).| Product Name | DYNLL1/2 Antibody (FL-89) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 1-89 mapping near the N-terminus of DYNLL1 of human origin |
| Modification Notes | Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. The highly conserved DYNLL proteins were originally identified as light chains for microtubule-based motor protein Dynein. In mammals there are two closely related isoforms expressed, DYNLL1 and DYNLL2 which share 93% sequence identity at the protein level. DYNLL1 (Dynein light chain 1) also designated, DLC8 or PIN (Protein inhibitor of neuronal nitric oxide synthase) has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition. Dimerization is required for NOS1 activity and DYNLL1 has been shown to destabilize the NOS1 dimer. Nitric oxide may be involved in several processes such as apoptosis, synaptogenesis and neuronal development; thus DYNLL1 is implicated in these processes as well. DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain. DYNLL2 (Dynein light chain 2) is subject to a unique alternative splicing event which is implicated in Myosin Va binding specificity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DYNLL1 and DYNLL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-89 mapping near the N-terminus of DYNLL1 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-89 mapping near the N-terminus of DYNLL1 of human origin |
| Entrez Gene | 8655 |
|---|
Application
Western blot analysis of DYNLL2 expression in non-transfected 293T: (A), mouse DYNLL2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of DYNLL1 expression in non-transfected 293T: (A), human DYNLL1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of DYNLL1/2 expression in HeLa whole cell lysate.
Application
Western blot analysis of DYNLL1/2 expression in 293 (A), human platelet (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of DYNLL1/2 expression in HeLa whole cell lysate (A) and mouse testis tissue extract (B).| Application Notes | recommended for detection of DYNLL1 and DYNLL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
