Application
Western blot analysis of ATP6E expression in non-transfected: (A) and mouse ATP6E transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ATP6E expression in K-562 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ATP6E expression in HeLa whole cell lysate (A) and mouse brain tissue extract (B).| Product Name | ATP6E Antibody (FL-226) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-226 ATP6E of human origin |
| Modification Notes | ATP6E, also known as V-ATPase E, is a vacuolar-type H+-ATPase (V-ATPase). V-ATPase is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. ATP6E controls acidification of the vacuolar system and provides the main proton-motive force. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ATP6E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-226 ATP6E of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-226 ATP6E of human origin |
| Entrez Gene | 529 |
|---|
Application
Western blot analysis of ATP6E expression in non-transfected: (A) and mouse ATP6E transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ATP6E expression in K-562 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ATP6E expression in HeLa whole cell lysate (A) and mouse brain tissue extract (B).| Application Notes | recommended for detection of ATP6E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
