Application
Western blot analysis of HGF expression in JAR whole cell lysate.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Product Name | HGF Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-170 of hepatocyte growth factor of human origin |
| Modification Notes | Hepatocyte growth factor, or HGF, is a pleiotropic growth factor variously designated as scatter factor, hematopoietin A and mammary growth factor (1,2). HGF is synthesized as a single chain, 728 amino acid precursor with a 29 amino acid signal peptide which is not present in the mature protein (3). Biologically active HGF is composed of a disulfide linked å chain and a ∫ chain, both of which are highly glycosylated (4). HGF exerts its biological effects through the HGF receptor, c-Met, which is expressed by normal hepatocytes, gastric and intestinal epithelium, ovarian and endometrial endothelium and in the basal layers of skin (4,5). While c-Met is not thought to be expressed in normal lung, thyroid or pancreatic tissue, c-Met has been detected in tumors originating from such tissue (6). The c-Met proto-oncogene encodes a 1408 amino acid glycoprotein that represents the prototypic member of a novel family of receptor tyrosine kinases (RTKs) that include Ron, Sea and Sex (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-170 of hepatocyte growth factor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-170 of hepatocyte growth factor of human origin |
| Entrez Gene | 3082 |
|---|
Application
Western blot analysis of HGF expression in JAR whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.| Application Notes | recommended for detection of HGF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
