Application
Western blot analysis of Flt-4 expression in HEL 92.1.7 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane and cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane and cytoplasmic localization.Application
Antibodies tested include Flt-4 (C-20): (A) and Flt-4 (M-20): (B).| Product Name | Flt-4 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Flt-4 of human origin |
| Modification Notes | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1–3), Flk-1 (also designated VEGF-R2) (4–6) and Flt-4 (also designated VEGF-R3) (7), putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Flt-4 p170 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Flt-4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Flt-4 of human origin |
| Entrez Gene | 2324 |
|---|
Application
Western blot analysis of Flt-4 expression in HEL 92.1.7 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane and cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane and cytoplasmic localization.
Application
Antibodies tested include Flt-4 (C-20): (A) and Flt-4 (M-20): (B).| Application Notes | recommended for detection of Flt-4 p170 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
