Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing membrane and cytoplasmic staining of respiratory epithelial cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of SC expression in HeLa (A), AN3 CA (B) and SW480 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.| Product Name | SC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 465-764 mapping at the C-terminus of Poly-IG receptor (PIGR) of human origin |
| Modification Notes | Polymeric IgA and IgM is produced and secreted by B cells in the lamina propria, which is beneath the mucosal lining of polarized epithelial cells. Polymeric immunoglobulin receptors, also designated pIgRs, are expressed on the basolateral surface of glandular epithelia and mediate transcellular transport of secretory immunoglobulin polymers across the epithelium. pIgR associates with secreted dimeric IgA and IgM molecules. During transcellular transport of these Ig polymers, pIgR undergoes proteolytic cleavage to generate a fragment called secretory component (SC), polymeric immunoglobulin receptor or poly-IG receptor. When immunoglobulin polymers associate with SC, they become resistant to enzymatic degradation during the transcytosis process. SC and the pIgR are crucial for proper mucosal immunity where they represent a molecular chaperone for polymeric Igs to remain intact and enter into body fluids. The human SC (pIgR) gene maps to chromosome 1q31-q41 and encodes a 764 amino acid protein. The receptor contains five units with homology to the variable (V) units of immunoglobulins and a transmembrane region that shares homology to certain immunoglobulin variable regions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PIGR and secretory component (SC) of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 465-764 mapping at the C-terminus of Poly-IG receptor (PIGR) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 465-764 mapping at the C-terminus of Poly-IG receptor (PIGR) of human origin |
| Entrez Gene | 5284 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing membrane and cytoplasmic staining of respiratory epithelial cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of SC expression in HeLa (A), AN3 CA (B) and SW480 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.| Application Notes | recommended for detection of PIGR and secretory component (SC) of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
