Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.Application
Western blot analysis of HLA-DRα expression in non-transfected 293T: (A), human HLA-DRα transfected 293T: (B) and NAMALWA (C) whole cell lysates.Application
Western blot analysis of HLA-DRα expression in non-transfected 293T: (A), human HLA-DRα transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of HLA-DRα expression in NAMALWA (A), BJAB (B) and Ramos (C) whole cell lysates.| Product Name | HLA-DRα Antibody (FL-254) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-254 representing full length HLA-DRα of human origin |
| Modification Notes | Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in mhc ii-a/b dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (∫-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HLA-DRα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-254 representing full length HLA-DR&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-254 representing full length HLA-DRα of human origin |
| Entrez Gene | 3122 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.
Application
Western blot analysis of HLA-DRα expression in non-transfected 293T: (A), human HLA-DRα transfected 293T: (B) and NAMALWA (C) whole cell lysates.
Application
Western blot analysis of HLA-DRα expression in non-transfected 293T: (A), human HLA-DRα transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of HLA-DRα expression in NAMALWA (A), BJAB (B) and Ramos (C) whole cell lysates.| Application Notes | recommended for detection of HLA-DRα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
