Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane staining of cells in seminiferous ducts.Application
Western blot analysis of Fap-1 expression in SW480 whole cell lysate.| Product Name | Fap-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of Fap-1 of human origin |
| Modification Notes | In contrast to the growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally-related receptors containing a conserved “death domain” belonging to the TNF receptor superfamily. Putative downstream receptors of FAS include TRADD, FADD and RIP. A novel protein tyrosine phosphatase, Fap-1 (for FAS-associated phosphatase) (originally designated PTP-BAS), has been shown to associate with the carboxy terminus fifteen amino acids of FAS. Three isoforms of the protein result from alternative RNA splicings, the longest of which encodes a protein 2485 amino acids in length. Although lacking a transmembrane region, Fap-1 does contain a membrane-binding domain, similar to that found in cytoskeleton-associated proteins such as ezrin. Fap-1 does not seem to associate with CD40 or death domain proteins such as TNF-RI and TNF-RII. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Fap-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of Fap-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of Fap-1 of human origin |
| Entrez Gene | 5783 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane staining of cells in seminiferous ducts.
Application
Western blot analysis of Fap-1 expression in SW480 whole cell lysate.| Application Notes | recommended for detection of Fap-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
